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MyBioinfo
Tool Box
Genome Cutter
Reverse Complement
DNA to protein translation
Restriction enzyme digest of DNA
Melting Temperature (Tm) Calculation of DNA
DNA sequence manipulation
Sequence Alignment
Map Your Sequence to the Genome
Melting Temperature (Tm) Calculation (DNA nearest-neighbor)
Primer
(6-50 bases):
Basic Tm
Degenerated nucleotides are allowed
Base-Stacking Tm
Degenerated nucleotides are NOT allowed
Primer concentration:
nM
Salt concentration:
mM
Mg
2+
concentration:
mM
Source code is freely downloable at biophp.org
